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Titlebook: Cell Imaging Techniques; Methods and Protocol Douglas J. Taatjes,Jürgen Roth Book 2013Latest edition Springer Science+Business Media, LLC 2

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书目名称Cell Imaging Techniques
副标题Methods and Protocol
编辑Douglas J. Taatjes,Jürgen Roth
视频video
概述Updates the successful previous edition with all new content.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supp
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Imaging Techniques; Methods and Protocol Douglas J. Taatjes,Jürgen Roth Book 2013Latest edition Springer Science+Business Media, LLC 2
描述.Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of .Cell Imaging Techniques: Methods and Protocols. expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters  covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, .Cell Imaging Techniques: Methods and Protocols, Second Edition. is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility..
出版日期Book 2013Latest edition
关键词autophagy; colocalization analysis; electron microscopy; endocytosis; intravital microscopy; light sheet
版次2
doihttps://doi.org/10.1007/978-1-62703-056-4
isbn_softcover978-1-4939-6246-4
isbn_ebook978-1-62703-056-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2013
The information of publication is updating

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https://doi.org/10.1007/978-981-99-6434-5pter, the epi-fluorescence light path is introduced and the various components are discussed in detail. Recommendations are made for incident lamp light sources, excitation and emission filters, dichroic mirrors, objective lenses, and charge-coupled device (CCD) cameras in order to obtain the most s
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SpringerBriefs in Environmental Sciencen. Therefore, the specimen must be monitored for viability and health before, during, and after imaging sessions. Methods for monitoring cell viability and health will be discussed in this chapter. Another key to successful live-cell imaging is to minimize light exposure as much as possible. A summa
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https://doi.org/10.1007/978-981-99-6434-5 in the same region via multifluorescence allows for the analysis of differential protein expression. The defining step of multifluorescence labeling is the selection of primary antibodies from different host species. In addition, species-appropriate secondary antibodies must also be conjugated to d
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Obligation and Healthcare Reforms in China,on-fluorescent nanoparticle probes can be identified and tracked dynamically inside crowded living cells with either differential interference contrast (DIC) microscopy or planar illumination microscopy (PIM). The translational and rotational dynamics of anisotropic nanoparticles can be readily extr
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